BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 4

Peptides:

Sequences	Affinity Values
HAPRWHL(2) HAPRWHW(1) HAPRYHY(1) HSPRWHL(1) HSPRYHM(1)	[1.935/2.274] [1.421] [1.656] [2.177] [1.288]

Sequences Features:

Motif:

H-x-P-R-x-H

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/4

Reference:

PMID: 17881351

Target Name:

Anti-Ogawa O-antigen monoclonal antibody S-20-4

Template Name:

O-specific polysaccharide of LPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was measured at 450 nm with a kinetic microplate reader (Molecular Devices).

Brief Description:

Phage were incubated with the selection Ab in solution, followed by affinity capture of the Ab-phage complexes onto protein A/G-agarose. In panning, phage bound to resin were (nonelution) directly added to a culture of E. coli strain ER2738 for amplification. Amplified phage were purified by precipitating the phage with polyethylene glycol/NaCl.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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