General information
    ACovPid:ACoVP100304
    Trivial Name:P21S10
    Amino Acids Sequence:LDLTYEMLSLQQVVKLNEY
    Length:19
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    The Middle East respiratory syndrome coronavirus (MERS-CoV) :

    Source Description:S2 subunit of spike (S) protein of MERS-CoV
    Against Virus:

    The Middle East respiratory syndrome coronavirus (MERS-CoV)

    Inhibition Value Type:EC50
    Inhibitory Effect:0.89 ± 0.07
    Inhibitory Unit:µM
    Target Domain Name:
    Assay:Pseudotyped virus infection inhibition assay
    Assay Description:MERS-CoV pseudovirus was generated via cotransfection of 293T cells with the plasmid pNL4-3.luc.RE as well as the pcDNA3.1-MERS-CoV-S plasmid, as previously described(PMID: 23978242). Briefly, 293T cells (ATCC, Manassas, VA) were co-transfected with 20 µg of plasmid encoding Env-defective, luciferase-expressing HIV-1 (pNL4-3.luc.RE) and 20 µg of rMERS-CoV-S plasmid, respectively, into a-T175 tissue culture flask using the calcium phosphate method. Cells were changed into fresh DMEM 8 h later. Supernatants were harvested 72 h post-transfection and used for single-cycle infection. The plasmids encoding vesicular stomatitis virus G protein (VSV-G-pcDNA3.1) and pcDNA3.1 vector were co-transfected with pNL4-3.luc.RE plasmid to generate VSV-G pseudovirus and pseudovirus without Env (Env-) as controls. Peptides at graded concentrations were mixed with the pseudovirus and then incubated for 1 h at room temperature. The mixture was added to Huh-7 or Calu-3 cells, fresh medium was added 12 h later, and the cells were incubated for an additional 48 h at 37 °C. Fluorescence was determined immediately using a luciferase kit (Promega) and an Ultra 384 luminometer (Tecan) after the addition of luciferase substrate (Promega).
    Anti-CoV activity in vivo:
    Reference:29442512
    Comment:For Q1020R-MERS-CoV pseudovirus
    3D structure:

    StructureACoVP100304

    Structure Experiment Verified:NO
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