| General information | |
| ACovPid: | ACoVP100292 |
| Trivial Name: | P21L10 |
| Amino Acids Sequence: | LDLTYEMLSLQQVVK-LUSPA-LNE-LUSPA-Y |
| Length: | |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | LUSPA: left uncyclized (S)-2-(4-pentenyl) alanine; |
| Peptide Source: | The Middle East respiratory syndrome coronavirus (MERS-CoV) : |
| Source Description: | S2 subunit of spike (S) protein of MERS-CoV |
| Against Virus: | |
| Inhibition Value Type: | EC50 |
| Inhibitory Effect: | 3.55 ± 0.2 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Cell–cell fusion assay |
| Assay Description: | MERS-CoV S protein-mediated cell–cell fusion was assessed, as described previously.(PMID: 25331705, 25451066) In brief, 293T cells expressing MERS-CoV S protein and enhanced GFP (EGFP) were used as the effector cells (293T/MERS/EGFP), and Huh-7 cells expressing the MERS-CoV receptor DPP4 were used as the target cells. 293T/EGFP cells, which express only EGFP, were used as negative control cells. Huh-7 cells were plated in a 96-well plate (5 × 10^4 per well) and cultured at 37 °C for 5 h. Inhibitors at the indicated concentrations were then added, followed by the addition of 293T/MERS/EGFP cells or 293T/EGFP cells (1 × 10^4 per well). After coculture at 37 °C for 4 h, the 293T/MERS/EGFP cells and 293T/EGFP cells fused or unfused with Huh-7 cells were counted under an inverted fluorescence microscope (Nikon Eclipse Ti–S). |
| Anti-CoV activity in vivo: | |
| Reference: | 29442512 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | |
| Similar Peptides: | ACoVP100286 ACoVP100291 ACoVP100285 ACoVP100141 ACoVP100070 |