How to use SSH2.0 web service

• Please input Fv fragment sequence in FASTA format or upload a .fasta file.Examples can be loaded by clicking the "Example".
• Click "Predict" to submit your job.

Notice:

• The heavy chain and light chain should be inputted respectively.If you have no knowledge about the variable domain.We provide a web-server AbRSA^[1] for antibody numbering and variable domain delimiting.
• The sequences only consist of heavy or light chains are allowed.
• Please do not submit sequences through text box and file box simultaneously.
• The ID of heavy chain and light chain in one antibody must be same. Otherwise they will be regarded as two antibodies.

The results are dispalyed in a table

• Number: the serial number of the query sequence.
• Name: the ID of the query sequence.
• Length: the full length of the query sequence(heavy chain+ light chain).
• Probability: this column represents the probability of the risk of hydrophobic interaction. The antibody will be predicted to have a high risk of hydrophobic interaction if the probability is 0.5 or higher.
• Result: this column shows the prediction result."0" means the submitted antibody has low risk of hydrophobic interaction and "1" means the submitted antibody has high risk of hydrophobic interaction

• Sometimes "Result" page reports "No matching records found" for the same inputting. Please try to refresh the page several times and submit your job again.
• If you have more questions, please contact hj@uestc.edu.cn