Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 999 |
| PDBID: | 4UTA |
| Chains: | IM_A |
| Organism: | dengue virus type 2, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/nature14130 |
| Antibody | |
| Antibody: | EDE1-C8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue 2 virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | P27914; P12823; |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4UTA_I|Chain C[auth H], D[auth I]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C8 HEAVY CHAIN|HOMO SAPIENS (9606) EVQLVESGGGLVQPGGSLRLSCSASGFTFSTYSMHWVRQAPGKGLEYVSAITGEGDSAFYADSVKGRFTISRDNSKNTLYFEMNSLRPEDTAVYYCVGGYSNFYYYYTMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPLEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Light Chain: M
Mutation: NULL
| >4UTA_M|Chain E[auth L], F[auth M]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C8 LIGHT CHAIN|HOMO SAPIENS (9606) RSEIVLTQSPATLSLSPGERATLSCRASQSISTFLAWYQHKPGQAPRLLIYDASTRATGVPARFSGSRSGTDFTLTISTLEPEDFAVYYCQQRYNWPPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >4UTA_A|Chain A, B|ENVELOPE GLYCOPROTEIN E|DENGUE VIRUS 2 (11060) MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFICKHSMVDRGWGNGCGLFGKGGIVTCAKFTCKKNMEGKIVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIKITPQSSTTEAELTGYGTVTMECSPRTGLDFNEMVLLQMEDKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIVGVEPGQLKLNWLRPLESRGPFEGKPIPNPLLGLDSTRTGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: THR52 GLU54 ASP56 SER57 ALA58 TYR60 LYS65 ASN102 PHE103 TYR104 TYR105 TYR106 M: SER0 GLN27 SER30 PHE32 TYR92 ASN93 TRP94 A: THR68 THR69 THR70 GLU71 SER72 ARG73 CYS74 GLN77 ASN83 GLU84 VAL97 ASP98 ARG99 TRP101 GLY102 ASN103 GLY104 CYS105 GLY106 ILE113 LYS246 LYS247 GLN248 ASP249 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
