Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 996 |
| PDBID: | 4UT9 |
| Chains: | JN_D |
| Organism: | dengue virus type 2, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/nature14130 |
| Antibody | |
| Antibody: | EDE1-C10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue 2 virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | P27914; P12823; |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >4UT9_J|Chain E[auth H], F[auth I], G[auth J], H[auth K]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C10|HOMO SAPIENS (9606) MAEVQLVESGAEVKKPGASVKVSCKASGYTFTSYAMHWVRQAPGQRLEWMGWINAGNGNTKYSQKFQDRVTITRDTSASTAYMELSSLRSEDTAIYYCARDKVDDYGDYWFPTLWYFDYWGQGTLVTVSSGTGGSGGGGSGGGG |
Light Chain: N
Mutation: NULL
| >4UT9_N|Chain I[auth L], J[auth M], K[auth N], L[auth O]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C10|HOMO SAPIENS (9606) SGGGASQSALTQPASVSGSPGQSITISCTGTSSDVGGFNYVSWFQQHPGKAPKLMLYDVTSRPSGVSSRFSGSKSGNTASLTISGLQAEDEADYYCSSHTSRGTWVFGGGTKLTVLAAADDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Antigen
Chain: D
Mutation: NULL
| >4UT9_D|Chain A, B, C, D|ENVELOPE GLYCOPROTEIN E|DENGUE VIRUS 2 (11060) MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFICKHSMVDRGWGNGCGLFGKGGIVTCAKFTCKKNMEGKIVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIKITPQSSTTEAELTGYGTVTMECSPRTGLDFNEMVLLQMEDKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIVGVEPGQLKLNWLRPLESRGPFEGKPIPNPLLGLDSTRTGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: LYS58 GLN64 ASP98 N: GLY29 PHE30 ASN31 TYR32 ASP50 SER93 ARG94 D: ASN67 THR68 THR69 THR70 GLU71 SER72 ARG73 CYS74 GLN77 SER81 LEU82 ASN83 GLU84 VAL97 ASP98 ARG99 TRP101 GLY102 ASN103 GLY104 CYS105 GLY106 ILE113 THR115 LYS246 LYS247 GLN248 ASP249 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
