Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 985 |
| PDBID: | 4UAO |
| Chains: | CB_A |
| Organism: | Plasmodium knowlesi, Rattus norvegicus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1371/journal.pone.0123567 |
| Antibody | |
| Antibody: | R31C2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Apical merozoite antigen 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >4UAO_C|Chain C|immunoglobulin R31C2 VH and CH1 regions|Rattus norvegicus (10116) EVQLVESGGGLVQPGRSLKLSCAASGFTFSNYYMAWVRQAPKKGLEWVATITTSGSRSYYPDSVKGRFTISRDNSESSLYLQMNSLESEDTATYYCARRGYGGYSEDFDYWGQGVMVTVSSAETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNSGALSSGVHTFPAVLQSGLYTLTSSVTVPSSTWSSQAVTCNVAHPASSTKVDKKIVPREC |
Light Chain: B
Mutation: NULL
| >4UAO_B|Chain B|immunoglobulin R31C2 light chain|Rattus norvegicus (10116) DIQMTQSPSSMSASLGDRVTITCQASQDIGNNLIWFQQKPGKSPRRMIYYATKLANGVPSRFSGSRSGSDYSLTIISLESEDMADYHCLQYKQFPLTFGSGTRLEIKRADAAPTVSIFPPSTEQLATGGASVVCLMNNFYPRDISVKWKIDGTERRDGVLDSVTDQDSKDSTYSMSSTLSLTKADYESHNLYTCEVVHKTSSSPVVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4UAO_A|Chain A|Apical merozoite antigen 1|Plasmodium knowlesi (5850) EFPIIERSIRMSNPWKAFMEKYDLERAHNSGIRIDLGEDAEVGNSKYRIPAGKCPVFGKGIVIENSKVSFLTPVATGAQRLKEGGFAFPNADDHISPITIANLKERYKENADLMKLNDIALCKTHAASFVIAEDQNTNYRHPAVYDEKEKTCYMLYLSAQENMGPRYCSPDSQNKDAMFCFKPDKNEKFDNLVYLSKNVRNDWENKCPRKNLGNAKFGLWVDGNCEEIPYVNEVEARSLRECNRIVFEASASDQPRQYEEELTDYEKIQEGFRQNNRDMIKSAFLPVGAFNSDNFKSKGRGYNWANFDSVNNKCYIFNTKPTCLINDKNFFATTALSHPQEVDNEFPGLEQKLISEEDLNSAVDHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP28 ASN31 TYR49 TYR50 LYS53 ARG66 SER67 GLY68 TYR91 LYS92 GLN93 PHE94 LEU96 C: THR28 SER30 ASN31 TYR32 TYR33 TRP47 THR50 THR52 SER53 SER55 ARG56 TYR58 ARG95 GLY96 TYR97 GLY98 GLY99 A: GLU81 GLY83 ASN84 PHE128 PRO129 ALA131 ASP132 HIS134 ILE135 ARG146 TYR147 GLU149 ASN150 PHE169 VAL170 ILE171 ALA172 GLU173 ASP174 GLN175 ASN176 THR177 HIS181 TYR196 GLN314 ASN315 ASN316 ARG317 ASP318 LYS321 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
