Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 981 |
| PDBID: | 4U6H |
| Chains: | AB_E |
| Organism: | Mus musculus, Vaccinia virus WR |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1128/JVI.01491-14 |
| Antibody | |
| Antibody: | M12B9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vaccinia virus L1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4U6H_A|Chain A, C|Heavy chain of murine anti-vaccinia L1 IgG2a antibody M12B9|Mus musculus (10090) QVQLQQSGAELVKTGASLRMSCKSSGYTFTRFWMHWIKQSPGQGLEWIGYINPSTGYTEYNQKFKDKATLTADRSSTTAYMQLISLTSGDSAVYYCARSDQTNYLFPYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: B
Mutation: NULL
| >4U6H_B|Chain B, D|Light chain of murine anti-vaccinia L1 IgG2a antibody M12B9|Mus musculus (10090) DVVMTQIPLSLPVSLGDQASISCRSSQSLVHSNGNTYLHWSLQKPGQSPNLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHDPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >4U6H_E|Chain E, F[auth J]|Protein L1|Vaccinia virus (10254) MGAAASIQTTVNTLSERISSKLEQEANASAQTKCDIEIGNFYIRQNHGCNLTVKNMCSADADAQLDAVLSAATETYSGLTPEQKAYVPAMFTAALNIQTSVNTVVRDFENYVKQTCNSSAVVDNKLKIQNVIIDECYGAPGSPTNLEFINTGSSKGNCAIKALMQLTTKATTQIAPKQVAGTGV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR28 THR30 ARG31 PHE32 TRP33 TYR50 ASN52 SER54 THR55 GLY56 TYR57 THR58 GLU59 ARG74 GLN101 THR102 ASN103 TYR104 B: ASN33 ASN35 TYR37 LYS55 ASP99 TRP101 E: GLU23 GLN24 GLU25 ALA26 ASN27 ALA28 SER29 GLN31 THR32 LYS33 ASP35 ILE36 SER58 ALA59 ASP60 ALA61 ASP62 VAL122 LYS125 LYS127 SER153 SER154 LYS155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
