Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 951 |
| PDBID: | 4RX4 |
| Chains: | AD_E |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.45 |
| Reference: | 10.1016/j.cell.2015.05.007 |
| Antibody | |
| Antibody: | 8ANC134 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade A Q842.d12 gp120 |
| Antigen mutation: | No |
| Durg Target: | P35961 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4RX4_A|Chain B[auth A], E[auth H]|8ANC134 Heavy chain|Homo sapiens (9606) QGQLVQSGGGVKKPGTSVTISCLASEYTFNEFVIHWIRQAPGQGPVWLGLIKRSGRLMTSYKFQDRLSLRRDRSTGTVFMELRGLRLDDTAVYYCARDGLGEVAPAYLYGIDAWGQGSTVIVTSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSCDK |
Light Chain: D
Mutation: NULL
| >4RX4_D|Chain C[auth D], F[auth L]|8ANC134 Light Chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQGLNFVVWYQQKGGQAPRLLIHGPTDRAPGVPDRFSARGSGTEFSLVISSVEPDDFALYYCQEYSSTPYNFGPGTRVERKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >4RX4_E|Chain A[auth E], D[auth G]|HIV-1 Clade A Q842.d12 gp120|Human immunodeficiency virus 1 (11676) VWKEAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTGGSAITQACPKVTFEPIPIHYCAPAGFAILKCKDEEFNGIGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVKIRCENITNNAKTIIVQLVNPVKINCTRPNNGGSGSGGDIRQAHCNVNRTEWNNTLHQVVEQLRKHFNKTINFANSTGGDLEITTHSFNCGGEFFYCNTTNLFNSTWNHTASMNSTESNDTIILPCRIKQIINMWQRVGQAMYAPPIRGVIRCESNITGLILTRDGGNTNSTRETFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: GLU26 PHE29 ASN30 GLU31 LEU50 LYS52 ARG53 SER54 GLY55 ARG56 LEU57 MET58 ARG73 SER74 THR75 GLY76 LEU97 GLY98 GLU99 D: ASN31 PRO51 TYR91 SER92 SER93 THR94 TYR96 E: GLU49 TRP96 LYS97 ASN98 ASN99 GLU102 LEU122 THR123 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 THR283 THR365 GLY366 GLY367 ASP368 GLU370 ILE371 ASN425 MET426 TRP427 GLN428 ARG429 VAL430 GLY431 GLN432 THR455 ARG456 ASP457 GLY472 GLY473 ASP474 ARG476 ASP477 ARG480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
