Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 943 |
| PDBID: | 4RGN |
| Chains: | HL_S |
| Organism: | Staphylococcus aureus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1074/jbc.M114.630715 |
| Antibody | |
| Antibody: | 14G8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Staphylococcal enterotoxin type B |
| Antigen mutation: | No |
| Durg Target: | P01552; P01552; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4RGN_H|Chain B, G[auth H]|14G8 heavy chain|Mus musculus (10090) EVNLIESGGDLVKPGGSLKLSCATSGFTFSAYGLSWVRQTPERRLEWVASISGGGSVYYPDSVKGRFTISRDTAGDILFLQMNSLRSEDSAIYYCVRDLYGDYVGRYAYWGQGTLVIVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >4RGN_L|Chain C, H[auth L]|14G8 light chain|Mus musculus (10090) DIVMTQSPATLSVTPGDRVSLSCRASQSIGDYLHWYQQKSHESPRLLINYASQSISGIPSRFSGSGSGSDFTLIINSVEPEDVGVYYCQNGHSFPYTFGGGTKLEIRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: S
Mutation: NULL
| >4RGN_S|Chain A, F[auth S]|Enterotoxin type B|Staphylococcus aureus (1280) GSEFGSESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTCMYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 SER30 ALA31 TYR32 SER52 GLY54 SER56 TYR58 THR73 TYR100 GLY101 ASP102 TYR103 VAL104 ARG106 L: ASP31 TYR32 TYR50 GLN53 SER54 SER56 GLY91 HIS92 PHE94 TYR96 S: PRO4 ASP5 PRO6 LYS7 PRO8 THR133 ARG135 PHE137 ASP139 GLY140 LYS141 ASN142 SER145 TYR186 LYS188 ILE190 ASN192 GLU193 LYS229 GLU231 TYR233 LEU234 THR235 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
