Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 942 |
| PDBID: | 4RGM |
| Chains: | HL_S |
| Organism: | Staphylococcus aureus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.69 |
| Reference: | 10.1074/jbc.M114.630715 |
| Antibody | |
| Antibody: | 20B1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Staphylococcal Enterotoxin B |
| Antigen mutation: | No |
| Durg Target: | P01552 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4RGM_H|Chain C, F[auth H]|20B1 heavy chain|Mus musculus (10090) QIQLVQSGPELKKPGETVRISCKASGYIFTIAGIQWVQKMPGRGLRWIGWINTHSGVPEYAEEFKGRFAFSLETSARTAYLQISNLKDEDTATYFCARIYYGNNGGVMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >4RGM_L|Chain B, E[auth L]|20B1 light chain|Mus musculus (10090) DIQMTQSPSSLSASLGERVSLTCRASQEISDYLTWLQQKPDGTIKRLIYVASSLDSGVPKRFSGSRSGSDYSLTISSLESEDFADYYCLQYANYPWTFGGGTKLEIRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: S
Mutation: NULL
| >4RGM_S|Chain A, D[auth S]|Enterotoxin type B|Staphylococcus aureus (1280) GSEFGSESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTCMYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 TYR91 ALA92 ASN93 TYR94 TRP96 H: ILE28 PHE29 THR30 ILE31 TRP50 ASN52 THR53 HIS54 SER55 GLY56 VAL57 LEU72 GLU73 THR74 SER75 ARG77 ILE99 TYR101 GLY102 ASN103 ASN104 VAL107 S: THR18 GLY19 LEU20 GLU22 ASN23 LYS25 VAL26 LEU27 ASP29 ASP30 ASN31 LYS57 LEU58 GLY59 ASN60 ASN88 TYR89 TYR90 TYR91 ARG110 LYS170 GLU176 PHE177 ASN178 ASN179 ASP206 LYS207 GLN210 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
