Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 935 |
| PDBID: | 4RDQ |
| Chains: | ML_D |
| Organism: | Gallus gallus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.85 |
| Reference: | 10.1038/nature13913 |
| Antibody | |
| Antibody: | 10D10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Bestrophin-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >4RDQ_M|Chain G, I, K, M, O|Fab antibody fragment, heavy chain|Mus musculus (10090) QVQLQQSGPELVRPGASVKMSCKASGYTFTNYWMHWVKQRPGQALEWIGMIDPSKSETTLNQKFRGKATLNVDKSSNTAYMQLSSLTSEDSAVYYCAREVYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: L
Mutation: NULL
| >4RDQ_L|Chain F, H, J, L, N|Fab antibody fragment, light chain|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRASENIYSYLTWYQQKQGKSPQLLVYNAKTLTEGVPSRFSGSGSGTQFSLKINSLQPEDFGGYFCQHHYGTPPTFGGGTKLEVKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: D
Mutation: NULL
| >4RDQ_D|Chain A, B, C, D, E|Bestrophin-1|Gallus gallus (9031) TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 TYR49 M: THR30 ASN31 TYR32 TRP33 ASP52 SER54 LYS55 GLU57 ARG98 GLU99 VAL100 TYR101 TYR102 ASP104 D: THR145 TYR148 LYS149 ARG150 PHE151 PRO152 SER153 GLU155 HIS156 ARG159 ALA160 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
