Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 93 |
| PDBID: | 1LK3 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Rattus norvegicus |
| Method: | XRD |
| Resolution (Å): | 1.91 |
| Reference: | 10.1016/s0969-2126(02)00791-8 |
| Antibody | |
| Antibody: | 9D7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-10 |
| Antigen mutation: | No |
| Durg Target: | P22301 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1LK3_H|Chain C[auth H], F[auth I]|9D7 Heavy Chain|Rattus norvegicus (10116) QVNLLQSGAALVKPGASVKLSCKASGYTFTDFYIHWVKQSHGKSLEWIGYINPNSGYTNYNEKFKNKATLTVDKSTSTGYMELSRLTSEDSANYSCTRGVPGNNWFPYWGQGTLVTVSSAETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNSGALSSGVHTFPAVLQSGLYTLTSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPR |
Light Chain: L
Mutation: NULL
| >1LK3_L|Chain B[auth L], E[auth M]|9D7 Light Chain|Rattus norvegicus (10116) DTVLTQPPALTVSPGEKLTISCKASESVTSRMHWYQQKPGQQPKLLIYKASNLASGVPARFSGSGSGTDFTLTIDPVEADDTAIYFCQQSWNGPLTFGAGTKLELKRADAAPTVSIFPPSTEQLATGGASVVCLMNNFYPRDISVKWKIDGTERRDGVLDSVTDQDSKDSTYSMSSTLSLTKADYESHNLYTCEVVHKTSSSPVVKSFNR |
Antigen
Chain: A
Mutation: NULL
| >1LK3_A|Chain A, D[auth B]|Interleukin-10|Homo sapiens (9606) MSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENGGGSGGKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP1 ARG31 LYS49 SER90 TRP91 ASN92 GLY93 PRO94 LEU95 H: TYR33 TRP47 TYR50 ASN52 ASN54 SER55 TYR57 THR58 ASN59 VAL100 PRO101 GLY102 ASN103 ASN104 A: MET39 PHE71 GLU74 GLU75 PRO78 GLN79 ASN82 GLN83 LYS125 PHE128 ASN129 LYS130 LEU131 GLN132 GLU133 LYS134 ILE136 TYR137 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
