Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 927 |
| PDBID: | 4RAU |
| Chains: | HG_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.74 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | RTO Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Platelet factor 4 |
| Antigen mutation: | No |
| Durg Target: | P02776 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4RAU_H|Chain B, E, H, K, N, Q, T, W|RTOFab heavy chain|Mus musculus (10090) EVKLVESGGGLVKPGGSLKLSCAASGFAFSRYDMSWVRQTPEKRLEWVATITSGDNYTYYPDSVKGRFTISRDNARNTLYLQMSRLRSEDTALYYCTRQGLLYYAMDYWGQGTSVNVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPS |
Light Chain: G
Mutation: NULL
| >4RAU_G|Chain A, D, G, J, M, P, S, V|RTOFab light chain|Mus musculus (10090) DIQMTQITSSLSASLGDRVTISCSASQGINNYLSWYRQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYFCQQFSKLPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: I
Mutation: NULL
| >4RAU_I|Chain C, F, I, L, O, R, U, X|Platelet factor 4|Homo sapiens (9606) EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: GLN29 GLY30 TYR34 TYR52 SER55 PHE93 SER94 LYS95 LEU96 TYR98 H: PHE29 ALA30 ARG33 TYR34 ASP35 THR54 SER55 GLY56 TYR61 LEU103 LEU104 TYR105 TYR106 I: GLU4 ASP5 ASP7 CYS10 LYS31 ALA32 GLY33 PRO34 HIS35 PRO37 THR38 ALA39 LEU55 GLN56 TYR60 ILE63 ILE64 LEU67 LEU68 GLU69 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
