Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 923 |
| PDBID: | 4R9Y |
| Chains: | HL_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 4.11 |
| Reference: | 10.1038/ncomms9277 |
| Antibody | |
| Antibody: | KKO Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Platelet factor 4 |
| Antigen mutation: | No |
| Durg Target: | P02776 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4R9Y_H|Chain D[auth H], H[auth N]|Platelet factor 4 antibody KKO heavy chain|Mus musculus (10090) VQLQQSGAELVKPGASVKLSCKASGYTFTNYFIYWVKQRPGQGLEWIGEINPRNGDTDFNEKFESRATLTVDKSSSTAYMQLSSLTSEDSAIYYCTRSPYGNNYGFTYWGQGTLVTVSAAKTTPPSVYPLAPGCGDAAGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >4R9Y_L|Chain C[auth L], G[auth M]|Platelet factor 4 antibody KKO light chain|Mus musculus (10090) DIQMIQSQKFMSTSVGDRVTVTCKASQNVGTNVAWYQQKPGQSPNALIYSASYRYSGVPDRFTGSGSGTDFTLTITNVQSEDLADYFCQQYNSYPLTFGTGTKLDLKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >4R9Y_B|Chain A, B, E[auth C], F[auth D]|Platelet factor 4|Homo sapiens (9606) EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: THR56 ASN57 TYR74 SER75 TYR78 ARG79 TYR80 SER81 TYR116 H: GLN2 GLY25 TYR26 THR27 ASN30 TYR31 ARG53 ARG97 PRO99 TYR100 GLY101 ASN102 ASN103 TYR104 THR107 B: GLY6 GLN9 CYS10 CYS12 VAL13 LYS14 THR15 ALA32 GLY33 PRO34 PRO37 THR38 ALA39 GLN40 CYS52 LEU53 ASP54 LEU55 GLN56 TYR60 LYS61 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
