Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 906 |
| PDBID: | 4QWW |
| Chains: | FE_A |
| Organism: | Bungarus fasciatus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1074/jbc.M114.603902 |
| Antibody | |
| Antibody: | 410 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | The acetylcholinesterase found in the venom of Bungarus fasciatus (BfAChE) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >4QWW_F|Chain D, F|Fab410 antibody heavy chain|Mus musculus (10090) EVQLVESGGGLVQPKGSLKLSCAASGFTFNTYAMHWVRQAPGKGLEWVARIRSKSNKYATHYADSVKDRFTISRDDSQTMLYLQMNNLKTEDTAMYYCVREGSYYDSSYGAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLYTLSSSVTVPSSPWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCIC |
Light Chain: E
Mutation: NULL
| >4QWW_E|Chain C, E|Fab410 antibody light chain|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSSVSYMYWYHQKPGSSPKPWIYRTSNLASGVPARFSGSGSGTSYSLSVSSVEAEDAATYYCQQYNSHPMTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4QWW_A|Chain A, B|Acetylcholinesterase|Bungarus fasciatus (8613) GRAGELKVSTQTGSVRGLSLPVLDGHVSAFLGIPFAEPPLGRMRFLRPEPVKPWQHVLDATSYKPACYQMVDTSYPGFQGTEMWNPNRGMSEDCLYLNIWVPSPRPKDAPVLVWIYGGGFYSGAASLDVYDGRFLTYTQNVILVSLSYRVGAFGFLGLPGSPEAPGNMGLLDQRLALQWIQNNIHPFGGNPRAVTVFGESAGAASVGMHLLSTQSRTLFQRAILQSGGPNAPWATVTPAESRGRAALLGKQLGCHFNNDSELVSCLRSKNPQELIDEEWSVLPYKSIFRFPFVPVIDGDFFPDTPEAMLSSGNFKETQVLLGVVKDEGSYFLIYGLPGFSKDNESLISRADFLEGVRMSVPHANDIATDAVVLQYTDWQDQDNREKNREALDDIVGDHNVICPVVQFANDYAKRNSKVYAYLFDHRASNLLWPPWMGVPHGYEIEFVFGLPLNDSLNYTPQEKELSRRMMRYWANFARTGNPTDPADKSGAWPTYTASQPQYVQLNTQPLATQPSLRAQICAFWNHFLPKLLNATVDPPRAD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: SER30 TYR31 TYR33 ARG49 TYR90 ASN91 SER92 HIS93 F: ASN30 THR31 ARG50 ARG52 LYS54 SER55 ASN56 LYS57 LYS67 GLU101 TYR104 TYR105 TYR109 GLY110 A: TYR68 MET70 VAL71 ASP72 THR73 SER74 TYR75 PRO76 GLY77 PHE78 GLN79 GLU82 ASN85 PRO86 MET90 GLN272 ASP276 GLU277 TRP279 LYS285 LYS341 ASP342 ASN343 LEU431 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
