Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 905 |
| PDBID: | 4QTI |
| Chains: | HL_U |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.jmb.2015.01.022 |
| Antibody | |
| Antibody: | 8B12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Urokinase plasminogen activator surface receptor(uPAR) |
| Antigen mutation: | No |
| Durg Target: | Q03405 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4QTI_H|Chain A[auth H]|anti-uPAR antibody, heavy chain|Mus musculus (10090) QVQLQQSGAELMKPGASVKLSCKAAGYTFTAYWIEWIRQRPGHGLEWIGEILPGSSSTNCNEMFKGKATFTADTSSNSAYMQLSSLTTEDSAIYYCTRDFSGDRSNLYFDVWGTGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >4QTI_L|Chain B[auth L]|anti-uPAR antibody, light chain|Mus musculus (10090) DIQMTQSPSSLSASLGERVSLTCRASQDIGSSLNWLQQEPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISRLESEDFVDYYCLQYATSPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: U
Mutation: NULL
| >4QTI_U|Chain C[auth U]|Urokinase plasminogen activator surface receptor|Homo sapiens (9606) LRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYRSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TRP33 GLU50 ASN59 ASP99 PHE100 SER101 GLY102 ASP103 ARG104 SER105 ASN106 TYR108 L: ILE2 ASP28 ILE29 GLY30 SER31 SER32 TYR49 ALA50 SER52 SER53 ARG66 TYR91 ALA92 THR93 SER94 TYR96 U: ARG58 SER88 ARG89 SER90 ARG91 TYR92 LEU93 GLU94 SER112 GLN114 ARG116 PRO118 ASN172 THR173 THR174 LYS175 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
