Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 904 |
| PDBID: | 4QO1 |
| Chains: | A_B |
| Organism: | Lama glama, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.92 |
| Reference: | 10.1093/nar/gku962 |
| Antibody | |
| Antibody: | Nb139 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | p53 DNA-binding domain |
| Antigen mutation: | No |
| Durg Target: | P04637 |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >4QO1_A|Chain A|Nb139 Nanobody against the DNA-binding domain of p53|Lama glama (9844) AQVQLQESGGGLVQAGGSLRLSCAASERTFSTYAMGWFRQAPGREREFLAQINWSGTTTYYAESVKDRTTISRDNAKNTVYLEMNNLNADDTGIYFCAAHPQRGWGSTLGWTYWGQGTQVTVSSGGGGSGGGKPIPNPLLGLDSTRTGHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >4QO1_B|Chain B|Cellular tumor antigen p53|Homo sapiens (9606) PLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR29 SER31 THR32 TYR33 ALA34 GLN51 ASN53 TRP54 THR57 HIS100 PRO101 GLN102 GLY104 TRP105 B: PRO98 SER99 TYR103 PRO152 MET160 LEU206 ASP208 ASN210 THR211 THR256 GLU258 ASP259 SER260 SER261 GLY262 ASN263 LEU264 LEU265 ARG267 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
