Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 899 |
| PDBID: | 4QEX |
| Chains: | IM_B |
| Organism: | Plasmodium falciparum, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 4.50 |
| Reference: | 10.1371/journal.ppat.1003390 |
| Antibody | |
| Antibody: | R217 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | malaria. P. falciparum EBA-175 (PfEBA-175) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4QEX_I|Chain C[auth H], F[auth I]|Antibody Heavy Chain|Mus musculus (10090) VQLQQSGPELVKPGTSVKISCKTSGYTFTENTMHWVKQSHGESLDWVGGINTDNGGTTYSQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCSTGYDAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: M
Mutation: NULL
| >4QEX_M|Chain B[auth L], E[auth M]|Antibody Light Chain|Mus musculus (10090) NIVLTQSPASLAVSLGQRATISCRASKGVDSYGNSFMHWYQQRPGQPPKLLIYLASHLESGVPARFSGSGSRTDFTLTIDPVEADDAATYYCQQNNEDPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTPGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKKGEFQHTGGRY |
Antigen
Chain: B
Mutation: N3Q/S50A/S195A/T206A
| >4QEX_B|Chain A, D[auth B]|Erythrocyte-binding antigen-175|Plasmodium falciparum (5833) GRQTSSNNEVLSNCREKRKGMKWDCKKKNDRSNYVCIPDRRIQLCIVNLAIIKTYTKETMKDHFIEASKKESQLLLKKNDNKYNSKFCNDLKNSFLDYGHLAMGNDMDFGGYSTKAENKIQEVFKGAHGEISEHKIKNFRKKWWNEFREKLWEAMLSEHKNNINNCKNIPQEELQITQWIKEWHGEFLLERDNRAKLPKSKCKNNALYEACEKECIDPCMKYRDWIIRSKFEWHTLSKEYETQKVPKENAENYLIKISENKNDAKVSLLLNNCDAEYSKYCDCKHTTTLVKSVLNGNDNTIKEKREHIDLDDFSKFGCDKNSVDTNTKVWECKKPYKLSTKDVCVPPRRQELCLGNIDRIYDKNLLMIKEHILAIAIYESRILKRKYKNKDDKEVCKIINKTFADIRDIIGGTDYWNDLSNRKLVGKINTNSNYVHRNKQNDKLFRDEWWKVIKKDVWNVISWVFKDKTVCKEDDIENIPQFFRWFSEWGDDYCQDKTKMIETLKVECKEKPCEDDNCKRKCNSYKEWISKKKEEYNKQAKQYQEYQKGNNYKMYSEFKSIKPEVYLKKYSEKCSNLNFEDEFKEELHSDYKNKCTMCPEVK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: PHE32 TYR49 LEU50 HIS53 ASN91 ASN92 ASP94 PHE96 I: THR28 THR30 GLU31 ASN32 THR33 HIS35 ASP53 ASN54 GLY95 TYR96 ASP97 MET99 B: GLU331 LYS333 PRO335 TYR336 LYS337 LEU338 THR340 LYS341 ASN417 LEU419 ARG422 LYS423 LYS439 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
