Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 894 |
| PDBID: | 4Q6I |
| Chains: | GF_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.65 |
| Reference: | 10.1126/scitranslmed.3008992 |
| Antibody | |
| Antibody: | 2D5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T cell surface glycoprotein CD4 |
| Antigen mutation: | No |
| Durg Target: | P01730 |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >4Q6I_G|Chain B, E, G, H|Heavy chain of murine 2D5 Fab|Mus musculus (10090) QVKLQQSGPELKKPGETVKISCKASGYTFTDYSMHWVKQAPGKGLKWLGRINTETGEAKYVDDFMGHLAFSLETSASTAYLQINNLKNEDTATYFCARYDGYSWDAMDYWGQGTSVIVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICT |
Light Chain: F
Mutation: NULL
| >4Q6I_F|Chain A, D, F, L|Light chain of murine 2D5 Fab|Mus musculus (10090) DIMLTQSPASLTVSLGQRATMSCRASQSVSTSRYSYMHWYQEKAGQPPQLLIKYASNLESGVPARFSGSGSGTDFTLNIHPVEAEDTATYYCQHSWEIPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: I
Mutation: NULL
| >4Q6I_I|Chain C, I, J, K|T-cell surface glycoprotein CD4|Homo sapiens (9606) MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKAS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: THR27 SER28 ARG29 TYR30 TYR32 TYR50 TRP92 GLU93 ILE94 TYR96 G: THR30 ASP31 TYR32 SER33 ARG50 THR52 GLU53 GLU56 TYR95 ASP96 GLY97 TYR98 SER99 I: HIS27 LYS29 SER31 ASN32 GLN33 ILE34 LYS35 LYS46 GLY47 PRO48 SER49 LYS50 ASN52 ASP53 GLU77 LYS90 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
