Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 89 |
| PDBID: | 1KXT |
| Chains: | B_A |
| Organism: | Sus scrofa, Camelus dromedarius |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1074/jbc.M202327200 |
| Antibody | |
| Antibody: | CABAMB7 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | porcine pancreatic alpha-amylase |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >1KXT_B|Chain B, D, F|IMMUNOGLOBULIN VHH FRAGMENT|Camelus dromedarius (9838) QVQLVASGGGSVQAGGSLRLSCAASGYTFSSYPMGWYRQAPGKECELSARIFSDGSANYADSVKGRFTISRDNAANTAYLQMDSLKPEDTAVYYCAAGPGSGKLVVAGRTCYGPNYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >1KXT_A|Chain A, C, E|ALPHA-AMYLASE, PANCREATIC|Sus scrofa (9823) QYAPQTQSGRTSIVHLFEWRWVDIALECERYLGPKGFGGVQVSPPNENIVVTNPSRPWWERYQPVSYKLCTRSGNENEFRDMVTRCNNVGVRIYVDAVINHMCGSGAAAGTGTTCGSYCNPGSREFPAVPYSAWDFNDGKCKTASGGIESYNDPYQVRDCQLVGLLDLALEKDYVRSMIADYLNKLIDIGVAGFRIDASKHMWPGDIKAVLDKLHNLNTNWFPAGSRPFIFQEVIDLGGEAIKSSEYFGNGRVTEFKYGAKLGTVVRKWSGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGSSILTFWDARLYKIAVGFMLAHPYGFTRVMSSYRWARNFVNGEDVNDWIGPPNNNGVIKEVTINADTTCGNDWVCEHRWREIRNMVWFRNVVDGQPFANWWDNGSNQVAFGRGNRGFIVFNNDDWQLSSTLQTGLPGGTYCDVISGDKVGNSCTGIKVYVSSDGTAQFSISNSAEDPFIAIHAESKL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR32 PRO33 TYR37 GLU44 CYS45 GLU46 LEU47 ARG50 PHE52 SER53 ASP54 SER56 ASN58 TYR59 ALA60 ASP61 LYS64 PRO96 GLY97 SER98 PRO100 ASN101 TYR102 A: LEU237 GLY238 GLY239 LYS257 ALA260 LYS261 THR264 TRP269 SER270 GLY271 GLU272 LYS273 TYR276 ASN279 GLY283 TRP284 GLY285 GLY308 GLY309 SER310 SER311 GLY413 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
