Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 887 |
| PDBID: | 4PLK |
| Chains: | HL_A |
| Organism: | Paslahepevirus balayani, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 4.00 |
| Reference: | 10.1038/cr.2015.34 |
| Antibody | |
| Antibody: | 8G12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis E Virus E2s domain (Genotype I) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4PLK_H|Chain D, F[auth H], J[auth I], L[auth J]|8G12 heavy chain|Mus musculus (10090) QLQQSGPELVKPGASVKISCKASGYTFTDFNMHWVKQSHGKSLEWIGYIYPYNGITGQNQKFKSKATLTVDNSSSSAYMELRSLTSEDSAVYYCARERFGVGNNYAWFTYWGQGTLVTVSSAKTTPPSVYPLAPGPVSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCTSKP |
Light Chain: L
Mutation: NULL
| >4PLK_L|Chain C, E[auth G], I[auth K], K[auth L]|8G12 light chain|Mus musculus (10090) DIQMTQSPASLSVSVGETVTITCRASENIYSNLVWYQQKQGKSPQVLVYAATNLPDGVPSRFSGSGSGTQYSLKINSLQSEDSGSYYCQHFWETPFTFGSGTKLEIKRADAAPTVSIFPPSSEKVLSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: A
Mutation: NULL
| >4PLK_A|Chain A, B, G[auth E], H[auth F]|Capsid protein|Hepatitis E virus (1678143) SRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQHSKTFFVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLAPPP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP1 ILE2 GLU27 ASN28 TRP92 GLU93 THR94 PRO95 H: TYR48 ILE55 THR56 GLY57 GLN58 GLN60 LYS63 GLY102 ASN103 ASN104 TYR105 A: THR476 ALA477 GLU479 SER497 GLU549 ALA550 GLY551 THR552 THR553 LYS554 THR586 SER587 LEU588 GLY589 ALA590 GLY591 PRO592 VAL593 SER594 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
