Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 871 |
| PDBID: | 4OII |
| Chains: | IM_B |
| Organism: | West Nile virus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1073/pnas.1322036111 |
| Antibody | |
| Antibody: | 22NS1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | NON-STRUCTURAL PROTEIN NS1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4OII_I|Chain D[auth H], F[auth I]|Heavy Chain of Fab fragment of 22NS1 Antibody|Mus musculus (10090) QVQLQQPGAELVKPGASVKLSCKASGYTFTSYWMHWVKLRPGQGFEWIGDINPNNGGPSYNEKFKRKATLTVDTSSSTAYMQLSSLTSEDSAVYYCTIDDGYRFGYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: M
Mutation: NULL
| >4OII_M|Chain C[auth L], E[auth M]|Light Chain of Fab fragment of 22NS1 Antibody|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYNAKTLADGVPSRFSASGSGTQYSLKINSLQPEDFGSYYCQHFWSTPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: B
Mutation: NULL
| >4OII_B|Chain A, B|NON-STRUCTURAL PROTEIN NS1|West Nile virus (11082) MASMRESNTTECDSKIIGTAVKNNLAIHSDLSYWIESRLNDTWKLERAVLGEVKSCTWPETHTLWGDGILESDLIIPVTLAGPRSNHNRRPGYKTQNQGPWDEGRVEIDFDYCPGTTVTLSESCGHRGPATRTTTESGKLITDWCCRSCTLPPLRYQTDSGCWYGMEIRPQRHDEKTLVQSQVNA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: HIS30 ASN31 TYR32 TYR49 ASN50 THR53 ASP56 PHE91 TRP92 SER93 THR94 ARG96 I: VAL2 GLY26 TYR27 THR28 THR30 SER31 TYR32 TRP33 HIS35 TRP47 ASP50 ASN53 SER58 ASP95 ASP96 GLY97 TYR98 ARG99 TYR102 B: ASP208 THR209 TRP210 TRP232 ASP234 GLY235 ILE236 LEU237 SER239 ASP240 ASN253 ARG256 ARG257 PRO258 GLY259 TYR260 LYS261 THR262 ASN264 GLN265 LEU287 GLU289 HIS293 ARG294 ARG314 SER315 THR317 MET333 ASN351 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
