Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 857 |
| PDBID: | 4NZT |
| Chains: | HL_M |
| Organism: | Mycoplasmoides genitalium G37, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1126/science.1246135 |
| Antibody | |
| Antibody: | CR9114 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein M TD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4NZT_H|Chain B[auth H]|CR9114 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKSSGGTSNNYAISWVRQAPGQGLDWMGGISPIFGSTAYAQKFQGRVTISADIFSNTAYMELNSLTSEDTAVYFCARHGNYYYYSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSAAGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >4NZT_L|Chain C[auth L]|CR9114 light chain|Homo sapiens (9606) QSALTQPPAVSGTPGQRVTISCSGSDSNIGRRSVNWYQQFPGTAPKLLIYSNDQRPSVVPDRFSGSKSGTSASLAISGLQSEDEAEYYCAAWDDSLKGAVFGGGTQLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: M
Mutation: NULL
| >4NZT_M|Chain A[auth M]|Protein M TD|Mycoplasma genitalium (243273) MGSSHHHHHHSSGLVPRGSHMSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL2 GLN3 SER25 GLY26 GLY27 ASN31 TYR32 ARG94 GLY96 ASP101 THR167 SER168 GLY169 L: THR14 PRO15 GLY16 GLN17 ARG18 GLN37 PHE39 THR42 ALA43 LYS45 LEU47 TYR49 ASP52 GLN53 ARG54 PRO55 SER56 VAL57 VAL58 PRO59 ASP60 ARG61 PHE62 SER76 GLY77 LEU78 GLN79 SER80 GLU81 ASP82 LEU106 GLY107 GLN108 PRO109 SER168 ASN170 ASN171 LYS172 M: ARG95 ARG99 ASN103 SER106 GLU107 ILE109 THR110 LYS114 ARG118 PRO119 TYR144 TYR158 SER160 THR161 GLU162 TYR163 ASN177 GLY178 THR179 LEU180 MET181 TYR187 ASP188 PHE191 ARG192 GLN193 ARG269 LYS319 ASN321 TYR338 LEU340 PHE341 ARG384 GLY388 TYR389 PHE390 ALA391 GLY392 GLY393 TYR394 ILE395 ASP396 GLU426 GLU427 TYR429 ARG438 VAL439 ASN440 GLU441 ASN442 TYR444 ALA447 ILE449 GLU453 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
