Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 854 |
| PDBID: | 4NNP |
| Chains: | HL_A |
| Organism: | Staphylococcus aureus subsp. aureus Mu50, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.69 |
| Reference: | 10.1016/j.str.2015.01.020 |
| Antibody | |
| Antibody: | FabC1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Apo Manganese ABC transporter MntC from Staphylococcus aureus |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4NNP_H|Chain C[auth H], D[auth X]|Heavy chain of antagonistic fab fragment|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNFSSSSIHWVRQAPGKGLEWVASIYSYSGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARQSSAEIESWYYYSGEAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >4NNP_L|Chain E[auth L], F[auth Y]|Light chain of antagonistic fab fragment|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSYYSSPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >4NNP_A|Chain A, B|Lipoprotein|Staphylococcus aureus subsp. aureus (158878) GTGGKQSSDKSNGKLKVVTTNSILYDMAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKALEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFIDNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGITPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFGEVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER32 TYR52 TYR54 SER55 TYR57 TYR59 SER106 TRP107 TYR108 TYR109 GLY112 GLU113 L: GLN27 SER91 TYR92 TYR93 SER94 SER95 PRO96 PHE97 A: GLU229 LYS230 GLN231 GLY232 THR233 PRO234 GLU235 MET237 ARG238 GLN239 ILE241 GLU242 LYS245 LYS260 ALA262 MET263 LEU266 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
