Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 853 |
| PDBID: | 4NIK |
| Chains: | B_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.jmb.2014.08.013 |
| Antibody | |
| Antibody: | F5 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 26S proteasome non-ATPase regulatory subunit 10 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4NIK_B|Chain B|Single-chain Fv fragment antibody|Homo sapiens (9606) MAEVQLVESGGSLVKPGGSLRLSCAASGFTFSNYSMNWVRQAPGKGLEWISSISGSSRYIYYADFVKGRFTISRDNATNSLYLQMNSLRAEDTAVYYCVRGVIDKFDYWGRGTLVTVSAGGGGSGGGGSGGGGSQSVLTQPASVSGSPGQSITISCAGTSSDVGGYNYVSWYQQHPGKAPKLMIYEDSKRPSGVSNRFSGSKSGNTASLTISGLQAEDEADYYCISYISSNTRLFGGGTKLAVLGAAASAHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4NIK_A|Chain A|26S proteasome non-ATPase regulatory subunit 10|Homo sapiens (9606) GSHMMEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: THR30 ASN33 TYR34 SER35 SER52 SER54 GLY55 SER56 ARG58 TYR59 ILE60 TYR61 VAL102 ILE103 ASP104 LYS105 GLY165 TYR166 ASN167 TYR168 TYR185 GLU186 TYR227 ILE228 SER229 SER230 ARG233 A: LYS90 HIS119 GLU120 VAL123 GLU127 GLY129 PRO132 LEU152 LYS153 HIS156 ILE157 LEU159 TYR160 TYR161 LYS162 GLU186 LYS189 LEU190 SER193 GLN194 MET223 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
