Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 850 |
| PDBID: | 4N9G |
| Chains: | MN_Z |
| Organism: | Macaca mulatta, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1038/nature12966 |
| Antibody | |
| Antibody: | 17HD9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | RSV-Presenting Epitope Scaffold FFL_001_C |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >4N9G_M|Chain A, E, G[auth H], I[auth M]|Antibody 17HD9, Heavy Chain|Macaca mulatta (9544) QVQLQESGPGLVKPSETLSLTCVVSGGSFSSYYWTWIRQSPGKGLEWIGEMNGNSGYTNYNPSLQSRVTISKDASKNQFSLKLTSLTAADTAVYYCARDAIVMVFTDMRGRVDVWGPGILVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: N
Mutation: NULL
| >4N9G_N|Chain B, F, H[auth L], J[auth N]|Antibody 17HD9, Light Chain|Macaca mulatta (9544) DIQMSQSPSSLSASVGDTVTITCRASQGISNYLAWYQQKPGKAPKSLIYYTSHLESGVPSRFSGSGSGTDFSLTISSLQPEDFATYYCQQHNSYPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECS |
Antigen
Chain: Z
Mutation: NULL
| >4N9G_Z|Chain C, D, K[auth Y], L[auth Z]|Epitope Scaffold rsv_1isea_FFL_001_C|synthetic construct (32630) GSRSDMRKDAERRFDKFVEAAKNKFDKFKAALRKGDIKEERRKDMKKLARKEAEQARRAVRNRLSELLSKINDMPITNDQKKLMSNDVLKFAAEAEKKIEALAADAEDKFTQGSWLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: TYR33 ASN52 SER55 TYR57 ASN59 VAL102 MET103 VAL104 PHE105 ASP107 ARG109 N: SER30 TYR32 TYR50 HIS91 ASN92 SER93 TYR94 ARG96 Z: SER65 LEU68 SER69 ASN72 ASP73 MET74 PRO75 ILE76 THR77 ASN78 ASP79 LYS81 LYS82 SER85 LEU89 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
