Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 846 |
| PDBID: | 4N90 |
| Chains: | DE_R |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1016/j.ccr.2014.04.028 |
| Antibody | |
| Antibody: | AMG-655 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Conatumumab(Phase-II) |
| Antigen | |
| Antigen: | Tumor necrosis factor receptor superfamily member 10B (DR5) |
| Antigen mutation: | No |
| Durg Target: | O14763 |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >4N90_D|Chain H[auth D], J[auth F], L[auth H]|Fab heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSQTLSLTCTVSGGSISSGDYFWSWIRQLPGKGLEWIGHIHNSGTTYYNPSLKSRVTISVDTSKKQFSLRLSSVTAADTAVYYCARDRGGDYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: E
Mutation: NULL
| >4N90_E|Chain G[auth E], I[auth G], K[auth I]|Fab light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQGISRSYLAWYQQKPGQAPSLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQFGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: R
Mutation: NULL
| >4N90_R|Chain A[auth R], B[auth S], C[auth T]|Tumor necrosis factor receptor superfamily member 10B|Homo sapiens (9606) TQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLY28 ILE29 SER30 ARG31 SER32 TYR33 SER68 GLY69 PHE92 GLY93 SER94 TRP97 D: GLY32 ASP33 PHE35 HIS52 HIS54 SER56 THR58 THR59 TYR60 GLY102 GLY103 ASP104 TYR107 R: LYS45 TYR46 GLY47 GLN48 ARG65 CYS66 ASP67 SER68 GLU70 VAL71 GLU72 PRO75 GLU87 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
