Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 843 |
| PDBID: | 4N1H |
| Chains: | D_C |
| Organism: | Bacillus licheniformis, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | cAb-F11N VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BlaP beta-lactamase from Bacillus licheniformis |
| Antigen mutation: | No |
| Durg Target: | P00808 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4N1H_D|Chain B, D|Camelid heavy-chain antibody variable fragment cAb-F11N|Lama glama (9844) QVQLQESGGGLVQAGASLKLSCAASGRTFSSYAMGWFRQAPGKEREFVAAISRSGGDTKYADSVKGRFAISRDNDKNTVWLRMNSLKPEDTAVYYCAATTYASLSDTYIGEHIYDDWGQGTQVTVSSHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >4N1H_C|Chain A, C|Beta-lactamase|Bacillus licheniformis (1402) TEMKDDFAKLEEQFDAKLGIFALDTGTNRTVAYRPDERFAFASTIKALTVGVLLQQKSIEDLNQRITYTRDDLVNYNPITEKHVDTGMTLKELADASLRYSDNAAQNLILKQIGGPESLKKELRKIGDEVTNPERFEPELNEVNPGETQDTSTARALVTSLRAFALEDPGKLPSEKRELLIDWMKRNTTGDALIRAGVPDGWEVADKTGAASYGTRNDIAIIWPPKGDPVVLAVLSSRDKKDAKYDDKLIAEATKVVMKALNMNGKGPHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ARG53 LYS59 THR99 THR100 TYR101 ALA102 SER103 ASP106 THR107 TYR108 ILE109 GLY110 HIS112 ILE113 ASP115 C: GLY143 GLU146 SER147 LYS149 LYS150 ARG153 LYS154 GLU158 ASN161 GLU163 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
