Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 84 |
| PDBID: | 1KXQ |
| Chains: | H_A |
| Organism: | Sus scrofa, Camelus dromedarius |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.1074/jbc.M202327200 |
| Antibody | |
| Antibody: | CABAMD9 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | porcine pancreatic alpha-amylase |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >1KXQ_H|Chain E, F, G, H|antibody VHH fragment CABAMD9|Camelus dromedarius (9838) QVQLVESGGGSVQAGGSLSLSCAASTYTDTVGWFRQAPGKEREGVAAIYRRTGYTYSADSVKGRFTLSQDNNKNTVYLQMNSLKPEDTGIYYCATGNSVRLASWEGYFYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >1KXQ_A|Chain A, B, C, D|alpha-amylase, pancreatic|Sus scrofa (9823) QYAPQTQSGRTSIVHLFEWRWVDIALECERYLGPKGFGGVQVSPPNENIVVTNPSRPWWERYQPVSYKLCTRSGNENEFRDMVTRCNNVGVRIYVDAVINHMCGSGAAAGTGTTCGSYCNPGSREFPAVPYSAWDFNDGKCKTASGGIESYNDPYQVRDCQLVGLLDLALEKDYVRSMIADYLNKLIDIGVAGFRIDASKHMWPGDIKAVLDKLHNLNTNWFPAGSRPFIFQEVIDLGGEAIKSSEYFGNGRVTEFKYGAKLGTVVRKWSGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGSSILTFWDARLYKIAVGFMLAHPYGFTRVMSSYRWARNFVNGEDVNDWIGPPNNNGVIKEVTINADTTCGNDWVCEHRWREIRNMVWFRNVVDGQPFANWWDNGSNQVAFGRGNRGFIVFNNDDWQLSSTLQTGLPGGTYCDVISGDKVGNSCTGIKVYVSSDGTAQFSISNSAEDPFIAIHAESKL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLN1 VAL2 THR26 TYR27 THR28 ASP29 TYR49 ARG50 ARG51 THR52 GLY53 TYR54 THR55 TYR56 LYS62 GLN69 ASP70 ASN71 ASN72 LYS73 ASN74 ASN97 VAL99 ARG100 LEU101 ALA102 PHE108 TYR109 A: VAL51 THR52 ASN53 PRO54 TRP58 TRP59 GLN63 GLY104 SER105 GLY106 ALA107 ALA108 ALA144 SER145 GLY147 ILE148 GLU149 TYR151 GLN161 LEU162 VAL163 GLY164 LEU165 LYS200 GLU240 ASP300 HIS305 ASN347 PHE348 VAL349 ASN350 GLU352 VAL354 ASN355 ASP356 TRP357 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
