Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 833 |
| PDBID: | 4N1C |
| Chains: | AB_C |
| Organism: | Homo sapiens, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | 10.1073/pnas.1613477114 |
| Antibody | |
| Antibody: | VL12 VL-sdAb |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lysozyme C |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4N1C_A|Chain A, B|immunoglobulin variable light chain domain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDIASDLNWYQQKPGKAPKLLIYSDSYLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYMIPSTFGQGTKVEIKRA |
Light Chain: B
Mutation: NULL
| >4N1C_B|Chain A, B|immunoglobulin variable light chain domain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDIASDLNWYQQKPGKAPKLLIYSDSYLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYMIPSTFGQGTKVEIKRA |
Antigen
Chain: C
Mutation: NULL
| >4N1C_C|Chain C|Lysozyme C|Gallus gallus (9031) KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: GLN27 ALA30 SER31 ASP32 TYR91 TYR92 MET93 ILE94 B: GLN27 ASP28 ALA30 SER31 ASP32 TYR49 SER50 ASP51 TYR53 TYR91 TYR92 MET93 ILE94 C: ASN19 ARG21 GLY22 TYR23 SER24 ASN27 VAL99 GLY102 ASN103 GLY104 ASN106 ARG112 LYS116 GLY117 THR118 ASP119 VAL120 GLN121 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
