Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 832 |
| PDBID: | 4MWF |
| Chains: | AB_D |
| Organism: | Homo sapiens, Hepatitis C virus (isolate H) |
| Method: | XRD |
| Resolution (Å): | 2.65 |
| Reference: | 10.1126/science.1243876 |
| Antibody | |
| Antibody: | AR3C Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis C Virus Envelope glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4MWF_A|Chain A, E[auth H]|Fab AR3C heavy chain|Homo sapiens (9606) EVQLLEQSGAEVKKPGSSVKVSCETSGGTFDNYALNWVRQAPGQGLEWIGGVVPLFGTTRNAQKFQGRVTISDDKSTGTGHMELRSLRSEDTAVYYCVRSVTPRYCGGGFCYGEFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGS |
Light Chain: B
Mutation: NULL
| >4MWF_B|Chain B, F[auth L]|Fab AR3C light chain|Homo sapiens (9606) EIELTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTVSRLEPEDSAVYFCQQYYRSPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >4MWF_D|Chain C, D|Envelope glycoprotein E2|Hepatitis C virus (isolate H) (11108) QLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFDSSGCPERLASCGSSGCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGDNTLLCPTDCFRKHPEATYSRCGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP30 ASN31 TYR32 LEU53 PHE54 THR56 VAL96 THR97 PRO98 ARG99 B: SER31 ASN32 TYR49 GLY50 THR53 THR56 TYR91 D: ILE422 THR425 LEU427 ASN428 CYS429 ASN430 GLU431 SER432 LEU433 GLY436 LEU438 ALA439 LEU441 PHE442 TYR443 GLN444 LYS446 TRP529 ALA531 TYR613 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
