Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 825 |
| PDBID: | 4M5Z |
| Chains: | HL_A |
| Organism: | Influenza A virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.25 |
| Reference: | 10.1128/JVI.01388-13 |
| Antibody | |
| Antibody: | 5J8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4M5Z_H|Chain B[auth H]|Fab 5J8 heavy chain|Homo sapiens (9606) EVQLVESGPGLVKPSDILSLTCAVSGYSISSNYYWGWIRQPPGKGLEWIGSIYHSGSTYYKPSLESRLGISVDTSKNQFSLKLSFVSAADTAVYYCARHVRSGYPDTAYYFDKWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >4M5Z_L|Chain C[auth L]|Fab 5J8 light chain|Homo sapiens (9606) SYVLTQPPSVSVAPGETARISCGGNNIGTKVLHWYQQTPGQAPVLVVYDDSDRPSGIPERFSGSNSGNTATLTISRVEVGDEADYYCQVWDISTDQAVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >4M5Z_A|Chain A|Hemagglutinin HA1 chain|Influenza A virus (11320) GVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADAYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIIS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN32 TYR34 TYR52 TYR58 ARG97 SER98 GLY99 L: ASN27 GLY29 THR30 LYS31 VAL32 TYR49 ASP50 ASP51 ASP53 ASN66 ASN69 ILE93 SER94 A: TYR98 LYS133 GLY134 VAL135 THR136 ALA137 PRO140 ALA142 GLY143 ALA144 LYS145 TRP153 VAL155 ALA189 ASP190 GLN192 SER193 LEU194 LYS222 ASP225 GLN226 GLU227 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
