Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 824 |
| PDBID: | 4M3K |
| Chains: | B_A |
| Organism: | Bacillus licheniformis, Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.48 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | cAb-H7S VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-lactamase |
| Antigen mutation: | No |
| Durg Target: | P00808 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4M3K_B|Chain B|Camelid heavy-chain antibody variable fragment cAb-H7S|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGSISSITTMGWYRQDPGKGRELVALINSVGDTTYAGSVKGRFTISRDNAKNTVYLEMSSLKPEDTAVYYCNAFMSTNSGRTGSFWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4M3K_A|Chain A|Beta-lactamase|Bacillus licheniformis (1402) TEMKDDFAKLEEQFDAKLGIFALDTGTNRTVAYRPDERFAFASTIKALTVGVLLQQKSIEDLNQRITYTRDDLVNYNPITEKHVDTGMTLKELADASLRYSDNAAQNLILKQIGGPESLKKELRKIGDEVTNPERFEPELNEVNPGETQDTSTARALVTSLRAFALEDPGKLPSEKRELLIDWMKRNTTGDALIRAGVPDGWEVADKTGAASYGTRNDIAIIWPPKGDPVVLAVLSSRDKKDAKYDDKLIAEATKVVMKALNMNGKGPHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR32 THR33 TYR37 ARG45 GLU46 LEU47 LEU50 ASN52 VAL54 ASP56 THR57 THR58 LYS64 TYR94 ASN96 PHE98 SER100 GLY104 ARG105 THR106 GLY107 SER108 PHE109 TRP110 GLY111 A: GLN93 ARG94 ILE95 THR96 TYR97 THR98 ASP100 ASP101 LEU102 VAL103 ASN136 LEU137 LYS140 GLN141 GLY143 GLY144 PRO145 GLU146 SER147 GLU163 ARG164 PHE165 GLU166 PRO167 GLU168 GLN178 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
