Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 823 |
| PDBID: | 4LU5 |
| Chains: | IM_A |
| Organism: | Vaccinia virus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1371/journal.ppat.1005148 |
| Antibody | |
| Antibody: | A20G2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | vaccinia antigen A33R |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4LU5_I|Chain C[auth H], D[auth I]|Murine IgG2a A20G2 Heavy chain Fab domain|Mus musculus (10090) EVKLVESGGGLVQPGGSLKLSCATSGFTFSDYYIYWVRQTPEKRLEWVAYISNGGYKTYYPDTVKGRFTISRDNAKNILYLQMSRLKSEDTGIYYCARGMDYWGQGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: M
Mutation: NULL
| >4LU5_M|Chain E[auth L], F[auth M]|Murine IgG2a A20G2 Light chain Fab domain|Mus musculus (10090) DVVMTQTPLTLSVTIGQPASISCKSSQSLLYSNGKTYLNWLLQRPGQSPKRLIYLVSKLDSGVPDRFTGSGSGTDFTLKISRVEAEDLGIYYCVQGTHFPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4LU5_A|Chain A, B|A33R|Vaccinia virus (10245) MSTTQYDHKESCNGLYYQGSCYILHSDYQMFSDAAANCTAESSTLPNKSDVMITWLIDYVEDTWGSDGNPITKTTSDYQDSDVSQEVRKYFCVKTMN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: ASP31 TYR32 TYR33 TYR35 TYR50 SER52 ASN53 TYR56 LYS57 TYR59 ARG98 MET100 ASP101 M: TYR31 SER32 ASN33 LYS35 TYR37 ASN39 GLY96 THR97 HIS98 PHE99 TYR101 A: ASP115 TYR116 GLN117 MET118 PHE119 SER120 ASP121 SER154 ASP155 GLY156 ASN157 ASP165 GLN167 ASP168 SER169 ASP170 VAL171 SER172 GLN173 GLU174 VAL175 ARG176 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
