Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 820 |
| PDBID: | 4LSU |
| Chains: | HL_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1016/j.immuni.2013.04.012 |
| Antibody | |
| Antibody: | VRC-PG20 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade A/E 93TH057 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4LSU_H|Chain B[auth H]|HEAVY CHAIN OF ANTIBODY VRC-PG20|Homo sapiens (9606) QVHLMQSGTEMKKPGASVRVTCQTSGYTFSDYFIHWLRQVPGRGFEWMGWMNPQWGQVNYARTFQGRVTMTRDVYREVAYLDLRSLTFADTAVYFCARRMRSQDREWDFQHWGQGTRIIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >4LSU_L|Chain C[auth L]|LIGHT CHAIN OF ANTIBODY VRC-PG20|Homo sapiens (9606) QSALTQPPSVSGSPGQSITLSCTGASTSVAWYQQYADKAPRLIVFDGNKRPSDISSRFSGSQSGGTASLTISGLQSEDEAYYHCNAFEFFGGGTKLTVLSQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: G
Mutation: NULL
| >4LSU_G|Chain A[auth G]|HIV-1 CLADE A/E STRAIN 93TH057 GP120|Human immunodeficiency virus 1 (11676) VWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLQPCVKLTGGSVIKQACPKISFDPIPIHYCTPAGYVILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNGGSGSGGDIRKAYCEINGTKWNKVLKQVTEKLKEHFNNKTIIFQPPSGGDLEITMHHFNCRGEFFYCNTTQLFNNTCIGNETMKGCNGTITLPCKIKQIINMWQGTGQAMYAPPIDGKINCVSNITGILLTRDGGANNTSNETFRPGGGNIKDNWRSELYKYKVVQIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 PHE33 TRP47 TRP50 GLN53 TRP54 GLY55 GLN56 VAL57 ASN58 TYR59 ALA60 ARG61 THR62 GLN64 THR68 ARG71 VAL73 TYR74 L: SER27 THR31 PHE91 GLU96 PHE97 G: LYS97 LEU122 THR123 GLY124 THR257 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 SER365 GLY366 GLY367 ASP368 GLU370 ILE371 HIS375 ASN425 MET426 TRP427 THR430 GLY431 GLN432 THR455 ARG456 ASP457 GLY458 GLY459 ALA460 ASN461 THR463 ASN465 GLU466 THR467 ARG469 PRO470 GLY471 GLY472 GLY473 ASN474 ILE475 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
