Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 791 |
| PDBID: | 4KRP |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.82 |
| Reference: | 10.1016/j.str.2013.05.008 |
| Antibody | |
| Antibody: | 9G8 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P00533; Q504U8; P00533; Q504U8; |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4KRP_B|Chain B|Nanobody/VHH domain 9G8|Lama glama (9844) EVQLVESGGGLVQAGGSLRLSCAASGRTFSSYAMGWFRQAPGKEREFVVAINWSSGSTYYADSVKGRFTISRDNAKNTMYLQMNSLKPEDTAVYYCAAGYQINSGNYNFKDYEYDYWGQGTQVTVSSALEHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4KRP_A|Chain A|Epidermal growth factor receptor|Homo sapiens (9606) LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTTNGPKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLU1 VAL2 GLN3 ARG27 TYR32 TYR100 ILE102 ASN103 ASN106 TYR107 ASN108 ASP111 GLU113 TYR114 ASP115 TYR116 A: TYR292 GLU293 LYS304 GLU306 PRO308 ARG310 VAL312 THR339 SER340 LYS375 GLU376 THR378 GLU400 ILE401 ARG403 ARG405 LYS430 GLU431 SER433 LYS454 LYS455 LEU456 PHE457 GLY458 THR459 SER460 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
