Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 790 |
| PDBID: | 4KRP |
| Chains: | DC_A |
| Organism: | Homo sapiens, Lama glama, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.82 |
| Reference: | 10.1016/j.str.2013.05.008 |
| Antibody | |
| Antibody: | Cetuximab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P00533; Q504U8; P00533; Q504U8; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >4KRP_D|Chain D|Cetuximab heavy chain|Mus musculus (10090) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPK |
Light Chain: C
Mutation: NULL
| >4KRP_C|Chain C|Cetuximab light chain|Mus musculus (10090) DILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: A
Mutation: NULL
| >4KRP_A|Chain A|Epidermal growth factor receptor|Homo sapiens (9606) LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTTNGPKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLN27 ASN32 TYR50 ASN91 ASN92 ASN93 TRP94 THR96 D: ASN31 TRP52 SER53 GLY54 GLY55 ASN56 THR57 ASP58 TYR59 THR100 TYR101 TYR102 ASP103 TYR104 A: LEU348 PRO349 VAL350 ARG353 LEU382 GLN384 GLN408 HIS409 GLN411 PHE412 ALA415 VAL417 SER418 ILE438 SER440 GLY441 LYS443 ASN449 LYS465 ILE466 ILE467 SER468 ASN469 ARG470 GLY471 GLU472 ASN473 SER474 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
