Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 789 |
| PDBID: | 4KRO |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.05 |
| Reference: | 10.1016/j.str.2013.05.008 |
| Antibody | |
| Antibody: | EgA1 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P00533; Q504U8; P00533; Q504U8; |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4KRO_B|Chain B|Nanobody/VHH domain EgA1|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGRTFSSYAMGWFRQAPGKQREFVAAIRWSGGYTYYTDSVKGRFTISRDNAKTTVYLQMNSLKPEDTAVYYCAATYLSSDYSRYALPQRPLDYDYWGQGTQVTVSSLEHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4KRO_A|Chain A|Epidermal growth factor receptor|Homo sapiens (9606) LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN1 VAL2 ARG27 TYR32 THR99 TYR100 LEU101 SER102 SER103 ASP104 TYR105 SER106 ARG107 TYR108 LEU115 ASP116 ASP118 TYR119 A: TYR292 GLU293 MET294 GLU295 GLU296 LYS303 GLU306 PRO308 ARG310 THR339 LYS375 GLU376 GLU400 ILE401 ARG403 ARG405 LYS430 GLU431 SER433 LYS455 LEU456 PHE457 GLY458 THR459 SER460 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
