Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 778 |
| PDBID: | 4K94 |
| Chains: | HL_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1073/pnas.1317118110 |
| Antibody | |
| Antibody: | 19 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mast/stem cell growth factor receptor Kit |
| Antigen mutation: | No |
| Durg Target: | P10721 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4K94_H|Chain B[auth H]|Fab19 light chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNISSYSMHWVRQAPGKGLEWVASIYPYSGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYVYHALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >4K94_L|Chain A[auth L]|Fab19 heavy chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQWAVHSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECGGSDYKDDDDK |
Antigen
Chain: C
Mutation: NULL
| >4K94_C|Chain C|Mast/stem cell growth factor receptor Kit|Homo sapiens (9606) GAMVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR49 SER53 LEU54 TYR55 SER56 TRP91 VAL93 HIS94 H: VAL2 GLY26 PHE27 ASN28 SER30 SER31 TYR32 TYR52 TYR54 SER55 TYR57 TYR59 ARG98 TYR99 VAL100 TYR101 HIS102 ASP105 TYR106 C: PHE316 PRO317 MET318 ILE319 ASN320 THR321 THR322 VAL323 PHE324 VAL325 ASN326 GLY328 GLU329 ASN330 VAL331 ASP332 ILE334 GLU336 LYS358 GLU360 TYR362 PRO363 LYS364 SER365 GLU366 ARG372 GLU376 HIS378 THR380 ARG381 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
