Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 775 |
| PDBID: | 4JPW |
| Chains: | HL_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1016/j.cell.2013.03.018 |
| Antibody | |
| Antibody: | 12A21 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade A/E strain 73TH057 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4JPW_H|Chain B[auth H]|HEAVY CHAIN OF ANTIBODY 12A21|Homo sapiens (9606) SQHLVQSGTQVKKPGASVRVSCQASGYTFTNYILHWWRQAPGQGLEWMGLIKPVFGAVNYARQFQGRIQLTRDIYREIAFLDLSGLRSDDTAVYYCARDESGDDLKWHLHPWGQGTQVIVSPASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >4JPW_L|Chain C[auth L]|LIGHT CHAIN OF ANTIBODY 12A21|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTINCQAGQGIGSSLNWYQKKPGRAPKLLVHGASNLQRGVPSRFSGSGFHTTFTLTISSLQPDDVATYFCAVFQWFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >4JPW_G|Chain A[auth G]|HIV-1 CLADE A/E STRAIN 73TH057 GP120 WITH MUTATION H375S|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) VWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLQPCVKLTGGSVIKQACPKISFDPIPIHYCTPAGYVILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNGGSGSGGDIRKAYCEINGTKWNKVLKQVTEKLKEHFNNKTIIFQPPSGGDLEITMHSFNCRGEFFYCNTTQLFNNTCIGNETMKGCNGTITLPCKIKQIINMWQGTGQAMYAPPIDGKINCVSNITGILLTRDGGANNTSNETFRPGGGNIKDNWRSELYKYKVVQIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR31 ILE34 HIS35 TRP47 LEU50 VAL52 PHE54 GLY55 ALA56 VAL57 ASN58 TYR59 ALA60 ARG61 GLN62 GLN64 ARG71 ILE73 TYR74 ASP99 L: ASP1 ILE2 GLN27 ILE29 SER32 PHE91 GLN96 TRP97 G: TRP96 LYS97 LEU122 GLY124 THR257 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 THR283 SER365 GLY366 GLY367 ASP368 GLU370 ILE371 ASN425 MET426 TRP427 GLN428 GLY429 THR430 GLY431 GLN432 THR455 ARG456 ASP457 GLY458 GLY459 ALA460 ASN461 ASN462 ASN465 GLU466 THR467 ARG469 GLY472 GLY473 ASN474 LYS476 ARG480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
