Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 771 |
| PDBID: | 4JAN |
| Chains: | AB_I |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | 10.1038/nature12053 |
| Antibody | |
| Antibody: | CH103 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade C gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4JAN_A|Chain B[auth A], E[auth H]|ANTIGEN BINDING FRAGMENT OF HEAVY CHAIN of CH103|Homo sapiens (9606) QVQLQESGPGVVKSSETLSLTCTVSGGSMGGTYWSWLRLSPGKGLEWIGYIFHTGETNYSPSLKGRVSISVDTSEDQFSLRLRSVTAADTAVYFCASLPRGQLVNAYFRNWGRGSLVSVTAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: B
Mutation: NULL
| >4JAN_B|Chain C[auth B], F[auth L]|ANTIGEN BINDING FRAGMENT OF LIGHT CHAIN of CH103|Homo sapiens (9606) YELTQPPSVSVSPGQTATITCSGASTNVCWYQVKPGQSPEVVIFENYKRPSGIPDRFSGSKSGSTATLTIRGTQAIDEADYYCQVWDSFSTFVFGSGTQVTVLRQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: I
Mutation: NULL
| >4JAN_I|Chain A[auth G], D[auth I]|ENVELOPE GLYCOPROTEIN GP120 of HIV-1 clade C|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) VWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEMALGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTGGSTVTQACPKVSFDPIPIHYCAPAGYAILKCNNETFEGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAKEEIVIRSENLTDNAKTIIVHLKESVEIVCTRPGNGGSGSGGDTRQAHCNISEEKWNKTLQKVSKILQEHFPNKAIKFEPHSGGDLEITTHSFNCRGEFFYCNTTKLFNGTYNSTANSTGSVSNTTITLPCRIKQIVNMWQGVGRAMYAPPIKGNITCKSIITGLLLTRDGGNDDNDTETFRPGGGDMRDNWRSELYKYKVVEIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TYR33 TYR50 PHE52 GLU56 ARG97 GLY98 GLN99 B: SER27 THR31 ASN32 GLU50 ASN51 TYR52 LYS53 LYS66 SER67 GLY68 TRP91 SER93 I: ASP279 ASN280 PRO363 HIS364 SER365 GLY366 GLY367 ASP368 LEU369 GLU370 ILE371 THR455 ASP457 GLY458 GLY459 ASN460 ASP461 ASP462 ASN463 ARG469 PRO470 GLY472 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
