Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 758 |
| PDBID: | 4HT1 |
| Chains: | HL_T |
| Organism: | Homo sapiens, Oryctolagus cuniculus |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1371/journal.pone.0062697 |
| Antibody | |
| Antibody: | chimeric Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tumor necrosis factor ligand superfamily member 12 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4HT1_H|Chain B[auth H]|chimeric antibody Fab|Oryctolagus cuniculus (9986) QEQLVESGGGLVQPGGSLTLSCKASGFDFSTYYMSWVRQAPGKGLEWIGTVYVRQGTTYYASWLNGRFTISSDNAQNTVDLKMNSLTAADTATYFCAKGGYNYDDAFVIWGPGTLVTVSFASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >4HT1_L|Chain C[auth L]|chimeric antibody Fab|Oryctolagus cuniculus (9986) AIEMTQTPFSVSAAVGGTVTINCQASQNIYSNLAWYQQKPGQPPKLLMYTASYLASGVPSRFKGSGSRTEYTLTISGVQCADAATYYCQTAYYNSRPDTVAFGGGTEVVVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: T
Mutation: NULL
| >4HT1_T|Chain A[auth T]|Tumor necrosis factor ligand superfamily member 12|Homo sapiens (9606) MKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR31 TYR33 TYR52 ARG54 GLN55 TYR59 TYR101 ASN102 TYR103 ASP104 ASP105 L: TYR30 SER31 TYR49 THR50 TYR53 GLY66 SER67 ARG68 TYR92 TYR93 SER95 ARG96 PRO97 T: VAL31 ASP32 ILE55 GLU57 ILE59 GLU76 ALA79 VAL80 TYR81 LYS83 LEU87 ASP89 GLY90 VAL91 LEU92 ARG95 LEU97 GLU99 ARG124 GLY126 SER127 SER128 ARG130 ARG132 LEU134 PRO135 TRP136 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
