Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7497 |
| PDBID: | 6SGE |
| Chains: | D_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.50 |
| Reference: | 10.1016/j.chembiol.2019.08.009 |
| Antibody | |
| Antibody: | B6 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rho-related GTP-binding protein RhoB |
| Antigen mutation: | Yes |
| Durg Target: | P62745; P62745; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >6SGE_D|Chain B, D|Nanobody B6|Homo sapiens (9606) AVQLQASGGGFVQPGGSLRLSCAASGYGSTIETMGWFRQAPGKEREFVSAISRAPGPSQYYADSVKGRFTISRDNSKNTVYLQMNSLRAEDTATYYCAPINNRTMQDSMFLWNYWGQGTQVTVSSAAAENLYFQ |
Antigen
Chain: A
Mutation: Q63L
| >6SGE_A|Chain A, C|Rho-related GTP-binding protein RhoB|Homo sapiens (9606) MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQKRY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: THR33 PHE47 ALA50 SER52 ARG53 ALA54 PRO55 GLY56 PRO57 SER58 GLN59 TYR60 TYR61 ALA62 LYS66 ILE100 ASN101 ASN102 MET105 A: ARG5 LYS7 LYS27 VAL38 PHE39 GLU40 ASN41 TYR42 VAL43 GLU54 ALA56 TRP58 TYR66 ARG68 LEU69 PRO71 LEU72 SER73 PRO75 ASP76 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
