Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7478 |
| PDBID: | 6JHT |
| Chains: | ED_C |
| Organism: | Human hepatitis A virus Hu/Australia/HM175/1976 |
| Method: | EM |
| Resolution (Å): | 3.79 |
| Reference: | 10.1371/journal.pbio.3000229 |
| Antibody | |
| Antibody: | F9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis A virus (HAV) VP3 |
| Antigen mutation: | No |
| Durg Target: | P06441; P13901; P08617; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6JHT_E|Chain E|FAB Heavy Chain|Human hepatitis A virus Hu/Australia/HM175/1976 (12098) EVKLVESGGGLVKPGGSLKLSCAASAFTITTYGMSWVRQTPEKRLEWVATITAGGSYTYYPDSVKGRFTISRDNAKNTLYLQMSSLRSGDTAMYYCARKVTSVAEYYFDYWGQGTTLTVSSPKTTPPSVYPLAPASASTAASMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: D
Mutation: NULL
| >6JHT_D|Chain D|FAB Light Chain|Human hepatitis A virus Hu/Australia/HM175/1976 (12098) DIVLTQSPAIMSASPGEKVTMTCSATSGLSYIHWYQQKSGTSPKRWIYDTSKLAFGAPARFSGSGSGTSYSLTISSMEAEDAATYYCQQWDVNPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >6JHT_C|Chain C|VP3|Human hepatitis A virus Hu/Australia/HM175/1976 (12098) MMRNETRVSTTENVVNLSNYEDARAKMSFALDQEDWKSDPSQGGGIKITHFTTWTSIPTLAAQFPFNASDSVGQQIKVIPVDPYFFQMTNTNPDQKCITALASICQMFCFWRGDLVFDFQVFPTKYHSGRLLFCFVPGNELIDVTGITLKQATTAPCAVMDIAGVQSTLRFRVPWISDTPYRVNRYTKEAHQKGEYTAIGKLIVYCYNRLTSPSNVAHHVRVNVYLSAINLECFAPLYHAMDVTTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ARG45 TYR48 LYS52 LEU53 ALA54 PHE55 E: THR28 THR30 THR31 TYR32 GLY33 THR52 ALA53 GLY54 SER56 TYR57 ARG98 LYS99 VAL100 THR101 SER102 VAL103 ALA104 TYR106 TYR107 ASP109 TYR110 C: ALA68 SER69 ASP70 SER71 VAL72 GLY73 GLN74 GLN75 ILE76 LYS77 VAL78 ILE142 ASP143 VAL144 THR145 GLY146 ILE147 THR148 LEU149 LYS150 ARG209 HIS218 THR245 GLN246 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
