Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7475 |
| PDBID: | 6JHQ |
| Chains: | DE_C |
| Organism: | Human hepatitis A virus Hu/Australia/HM175/1976, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.90 |
| Reference: | 10.1371/journal.pbio.3000229 |
| Antibody | |
| Antibody: | F4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis A virus (HAV) VP3 |
| Antigen mutation: | No |
| Durg Target: | P06441; P13901; P08617; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >6JHQ_D|Chain D|FAB Heavy Chain|Mus musculus (10090) EVKLVESGGGLVKPGGSLKLSCAASLFTHNNYGMSWVRQTPEKRLEWVATINSTASYTYYPDSVKGRFTISRDNAKNTLYLQMSSLRSGDTAIYYCARKNDTFSDYYFDYWGQGTTLTVSSPKTTPPSVYPLAPASASTAASMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: E
Mutation: NULL
| >6JHQ_E|Chain E|FAB Light Chain|Mus musculus (10090) DIVLTQSPAIMSASPGERVTMTCSAHVSTDYMHWYQQKSGTSPKRWIYDTSKLASTVPDRFSGSGSGTSYSLTISSMEAEDAATYYCQQWNNNAYTYGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >6JHQ_C|Chain C|VP3|Human hepatitis A virus Hu/Australia/HM175/1976 (12098) MMRNETRVSTTENVVNLSNYEDARAKMSFALDQEDWKSDPSQGGGIKITHFTTWTSIPTLAAQFPFNASDSVGQQIKVIPVDPYFFQMTNTNPDQKCITALASICQMFCFWRGDLVFDFQVFPTKYHSGRLLFCFVPGNELIDVTGITLKQATTAPCAVMDIAGVQSTLRFRVPWISDTPYRVNRYTKEAHQKGEYTAIGKLIVYCYNRLTSPSNVAHHVRVNVYLSAINLECFAPLYHAMDVTTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLU1 PHE27 THR28 ASN30 ASN31 TYR32 GLY33 ASN52 SER53 THR54 SER56 TYR57 ASN74 ALA75 ASN77 ARG98 ASN100 ASP101 THR102 PHE103 SER104 ASP105 TYR106 TYR107 ASP109 TYR110 E: TYR31 ARG45 TYR48 LYS52 LEU53 ALA54 SER55 C: PRO65 ASN67 ALA68 SER69 ASP70 SER71 VAL72 GLY73 GLN74 GLN75 ILE76 LYS77 VAL78 LEU141 ILE142 ASP143 VAL144 THR145 GLY146 ILE147 THR148 LEU149 LYS150 LYS201 TYR207 ARG209 THR245 GLN246 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
