Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7450 |
| PDBID: | 6H7J |
| Chains: | D_B |
| Organism: | Escherichia coli K-12, Meleagris gallopavo, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1126/science.aau5595 |
| Antibody | |
| Antibody: | 80 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | β 1 -adrenoceptor (β 1 AR) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >6H7J_D|Chain C, F[auth D]|Camelid antibody fragment Nb80|Lama glama (9844) SQVQLQESGGGLVQAGGSLRLSCAASGSIFSINTMGWYRQAPGKQRELVAAIHSGGSTNYANSVKGRFTISRDNAANTVYLQMNSLKPEDTAVYYCNVKDYGAVLYEYDYWGQGTQVTVSS |
Antigen
Chain: B
Mutation: R30S/M52V/C78L/F261A/N272M/W292A
| >6H7J_B|Chain B[auth A], E[auth B]|Beta-1 adrenergic receptor|Meleagris gallopavo (9103) AAAKVMSLLMALVVLLIVAGNVLVIAAIGSTQRLQTLTNLFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQDPGCCDFVTNRAYAIASSIISFYIPLLIMIFVYLRVYREAKEQIRKIDRASKRKTSRVMAMKEHKALKTLGIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVAFNWLGYANSAMNPIIYCRSPDFRKAFKRLLAFPRKADRRLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER27 ILE28 PHE29 SER30 ILE31 THR33 ALA50 HIS52 SER53 GLY54 SER56 THR57 ASN58 ASN73 ALA74 ASN76 LYS98 ASP99 TYR100 GLY101 ALA102 VAL103 LEU104 TYR105 GLU106 TYR107 TYR109 B: THR76 ILE135 ARG139 ALA142 ILE143 THR144 SER145 PRO146 PHE147 GLN150 TYR227 VAL230 GLU233 ALA234 GLN237 ILE238 LYS240 ILE241 MET283 LYS284 GLU285 LYS287 ALA288 THR291 LEU292 ILE295 ILE342 TYR343 ARG345 SER346 PRO347 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
