Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7441 |
| PDBID: | 6FE4 |
| Chains: | J_E |
| Organism: | Escherichia phage 933W, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.3390/toxins10030108 |
| Antibody | |
| Antibody: | 113 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Shiga toxin-2a B subunit (Stx2aB) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: J
Mutation: NULL
| >6FE4_J|Chain F, G, H, I, J|Nb113|Vicugna pacos (30538) QVQLQESGGGLVQPGGSLRLSCAASGFTFSSYYMSWVRQAPGKGPEWVSGINTGGVGTRYADSVKGRFTISRDNAKNTLYLQMNSLKPEDTALYYCAIGEGGNRNYWGQGTQVTVSSHH |
Antigen
Chain: E
Mutation: NULL
| >6FE4_E|Chain A, B, C, D, E|Shiga-like toxin 2 subunit B|Enterobacteria phage 933W (10730) ADCAKGKIEFSKYNEDDTFTVKVDGKEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNNDLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: TYR33 VAL37 LYS43 PRO45 GLU46 TRP47 ASN52 GLY57 THR58 ARG59 GLY99 GLU100 GLY101 GLY102 ASN103 ARG104 TRP107 E: ALA20 LYS31 ASN33 ASP35 ASP36 THR37 THR39 TRP48 SER50 ARG51 LYS71 SER72 SER73 THR74 CYS75 GLU76 GLY78 SER79 GLY80 PHE81 ALA82 GLU83 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
