Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7432 |
| PDBID: | 6EWB |
| Chains: | EF_A |
| Organism: | Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.78 |
| Reference: | 10.1128/JVI.02174-18 |
| Antibody | |
| Antibody: | 10E9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | GII.4 Norovirus capsid VP1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6EWB_E|Chain E, G, H, J|Fab heavy chain|Mus musculus (10090) QVQLQQSGAELMKPGASVKISCTATGYTFITYWIQWVKQRPGHGLEWIGETLPGSGSTNYNEKFKGKATFTADTSSNTAYMQLSSLTSEDSAIYYCARIGRSNDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: F
Mutation: NULL
| >6EWB_F|Chain F, I, K, L|Fab light chain|Mus musculus (10090) GQIVLTQSPTIMSASPGEKVTMTCSASSSVDYMHWYQQKSGTSPKRWIYDTYKLASGVPARFSGSGSGTSYSLTINSMEAEDAATYYCQQWSSNPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >6EWB_A|Chain A, B, C, D|VP1|Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU (1241973) GPGSKPFSVPVLTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTTQLSPVNICTFRGDVTHITGSRNYTMNLASQNWNDYDPTEEIPAPLGTPDFVGKIQGVLTQTTRTDGSTRGHKATVYTGSADFAPKLGRVQFETDTDRDFEANQNTKFTPVGVIQDGGTTHRNEPQQWVLPSYSGRNTHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMDLDCLLPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLVIPPNGYFRFDSWVNQFYTLAPM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR32 TRP33 GLN35 TRP47 GLU50 ASN59 ARG98 ILE99 GLY100 ARG101 F: SER28 ASP30 TYR31 ASP49 THR50 TYR51 LYS52 GLY63 SER64 GLY65 SER66 GLY67 TRP90 SER91 ASN93 LEU95 A: PHE250 PRO253 SER255 ALA256 PHE257 ASP391 GLY392 GLY393 THR394 THR395 HIS396 ARG397 ASN398 GLN401 GLN402 ARG435 TYR444 PRO445 ASN446 MET447 ASP448 GLY503 GLN504 HIS505 ASP506 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
