Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 743 |
| PDBID: | 4G7V |
| Chains: | HL_S |
| Organism: | Homo sapiens, Ciona intestinalis |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1038/nsmb.2768 |
| Antibody | |
| Antibody: | 33F12_4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-sensor containing phosphatase |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4G7V_H|Chain A[auth H]|fragment antibody heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNVSSSSIHWVRQAPGKGLEWVASISSYYGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSYSWSYAIDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >4G7V_L|Chain B[auth L]|fragment antibody light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYFYWPITFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQAGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: S
Mutation: R139E
| >4G7V_S|Chain C[auth S]|Voltage-sensor containing phosphatase|Ciona intestinalis (7719) MRGSHHHHHHGVDDGRMEIPTTGVGRVQFRVRAVIDHLGMRVFGVFLIFLDIILMIIDLSLPGKSESSQSFYDGMALALSCYFMLDLGLRIFAYGPKNFFTNPWEVADGLIIVVTFVVTIFYTVLDEYVQETGADGLGELVVLARLLRVVRLARIFYSHQQMKASSRRTISQNKRRYRKDGFKLN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR55 SER101 TRP102 SER103 TYR104 ALA105 L: GLN27 SER28 SER30 SER31 ALA32 TYR49 SER50 ALA51 SER52 SER53 GLY66 SER67 GLY68 TYR91 PHE92 S: GLU144 TYR200 ASP204 GLU205 VAL207 GLN208 GLY211 ALA212 ASP213 GLY214 LEU215 GLY216 GLU217 LEU218 VAL219 VAL220 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
